P-101 A comprehensive profile of cell-free RNAs in seminal plasma reveals their noninvasive potential in male infertility

Abstract Study question Could the cell-free RNAs (cfRNAs) in seminal plasma be associated with male infertility and used as clinical biomarkers? Summary answer CfRNAs show different patterns men could serve diagnostic predictive factors infertility. What is known already Seminal a biological fluid containing large amounts of tissue-specific components secreted by organs reproductive system. Thus, has great potential sample for biomarker discovery noninvasive diagnostics disorders. Several metabolites, metal ions, proteins nucleotides are semen quality azoospermia forms subtypes. However, comprehensive transcriptome cfRNAs their value not clear. design, size, duration We applied previously developed library method polyadenylation ligation-mediated sequencing (PALM-Seq) to capture sequence from adult men, including 129 patients asthenozoospermia, 101 oligozoospermia, 102 (obstructive nonobstructive azoospermia) 107 healthy individuals. Participants/materials, setting, methods performed PALM-Seq on recruited individuals comprehensively profiled miRNA, tRNA, piRNA, lncRNA mRNA. In addition, we followed up outcome assisted technology (ART) during therapy. Main results role chance Hundreds miRNA tRNA detected all samples. Unlike other body biofluids, defined amount piRNA since highly expressed germ cells. Besides, showed group-specific pattern infertility, especially azoospermia. Differentially abundant mRNA oligozoospermia were mainly involved regulating mononuclear cell migration, thermogenesis development, respectively. The abundance was parameters quality, well sperm DNA fragmentation. At last, using collected omics data machine learning models, accurately classify subtypes successfully predict ART before treatment. Limitations, reasons caution only limited number this research more studies focused needed. Wider implications findings characterized kinds first time revealed changes Our contribute new insight into understanding mechanism provide invasive biopsies help improve treatment Trial registration Not applocable